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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472
EISSN: 1735-2630
Vol. 6, No. 3, 2009, pp. 443-450
Bioline Code: st09049
Full paper language: English
Document type: Research Article
Document available free of charge

International Journal of Environment Science and Technology, Vol. 6, No. 3, 2009, pp. 443-450

 en Substrate inhibition kinetics of phenol degradation by Pseudomonas fluorescence check for this species in other resources from steady state and wash-out data
Agarry, S. E.; Audu, T. O. K. & Solomon, B. O.

Abstract

The present study investigated the phenol utilization kinetics of a pure culture of an indigenous Pseudomonas fluorescence check for this species in other resources under steady state and non-steady state (washout) conditions. Steady states of a continuous culture with an inhibitory substrate was used to estimate kinetic parameters under substrate limitation (chemo stat operation) Pure cultures of an indigenous Pseudomonas fluorescence were grown in continuous culture on phenol as the sole source of carbon and energy at dilution rates of 0.010 0.20/h. Using different dilution rates, several steady states were investigated and the specific phenol consumption rates were calculated. In addition, phenol degradation was investigated by increasing the dilution rate above the critical dilution rate (washout cultivation). The results showed that the specific phenol consumption rate increased with increased dilution rate at steady state and phenol degradation by Pseudomonas fluorescence can be described by simple substrate inhibition kinetics under substrate limitation but cannot be described by simple substrate inhibition kinetics under washout cultivation. Fitting of the steady state data from continuous cultivation to various inhibition models resulted in the best fit for Haldane, Yano and Koga (2), Aiba and Teissier kinetic inhibition models. The rsmax value of 0.229 mg/mg/h obtained from the inhibition model equations was comparable to the experimentally calculated rsmax value of 0.246 mg/mg/h obtained under washout cultivation. Therefore, the biokinetic constants evaluated using these models showed good tolerance and growth of the indigenous organism.

Keywords
Bioreactor; Continuous cultivation; Kinetic parameters; Primary culture; Secondary culture; Washout cultivation

 
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