The enzyme urease drives the hydrolysis of urea leading to the release of ammonium ions and bicarbonate; in the presence of calcium, the rise in pH leads to increased calcium carbonate saturation and the subsequent precipitation of calcite. Although such alkalinizing ureolysis is widespread in nature, most studies have focussed on bacteria (i.e. indigenous communities or urease-active
Sporosarcina pasteurii
) for calcite precipitation technologies. In this study, urease-active jack bean meal (from the legume
Canavalia ensiformis
) was used to drive calcite precipitation. The rates of ureolysis (
kurea), determined from measured NH
4 +, enabled a direct comparison to microbial ureolysis rates reported in literature. It is also demonstrated that a simple single reaction model approach can simulate calcite precipitation very effectively (3-6 % normalised root-mean-square deviation). To investigate the reduction of permeability in porous media, jack bean meal (0.5 g L
-1) and solutions (400 mM urea and CaCl
2) were simultaneously pumped into a borosilicate bead column. One-dimensional magnetic resonance profiling techniques were used, non-invasively, for the first time to quantify the porosity changes following calcite precipitation. In addition, two-dimensional slice selective magnetic resonance images (resolution of ~0.5 × 1.0 mm) revealed that the exact location of calcite deposition was within the first 10 mm of the column. Column sacrifice and acid digestion also confirmed that 91.5 % of calcite was located within the first 14 mm of the column. These results have important implications for the design of future calcite precipitation technologies and present a possible alternative to the well known bacterial approaches.
