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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 3, No. 1, 2006, pp. 115-128
Bioline Code: tc06013
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 3, No. 1, 2006, pp. 115-128

 en EXPERIMENTAL DESIGN OPTIMIZATION FOR SCREENING RELEVANT FREE PHENOLIC ACIDS FROM VARIOUS PREPARATIONS USED IN BURKINA FASO FOLK MEDICINE
Martin Kiendrebeogo, Luc Choisnard, Charles E. Lamien, Aline Meda, Denis Wouessidjewe, Odile G. Nacoulma

Abstract

Experimental design methodology was used to optimize the HPLC separation of various relevant phenolic acids from an artificial mixture. The effect of four characteristic factors of the HPLC procedure on the Chromatographic Response Function was investigated by a Central Composite Face-Centred Design and Multi Linear Regression fitting. The eight phenolic acids were successfully separated. The optimal HPLC setting condition was applied to rapid screening of free phenolic acids from Striga hermonthica check for this species in other resources (Del.) Benth. (Scrophulariaceae), Guiera senegalensis check for this species in other resources J. F. Gmel (Combretaceae) and unifloral honey from Acacia sp. (Mimosaceae). None of reference phenolic acid was identified in S. hermonthica extract when some of them were detected in G. senegalensis and honey extracts. This work is considered as a first step for a largest phenolic acids screening from various preparations used in Burkina Faso folk medicine to justify some of their properties or potentialities.

Keywords
Central Composite Face-Centred Design, Chromatographic Response Function, Phenolic acids

 
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