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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 4, No. 4, 2007, pp. 476 – 480
Bioline Code: tc07063
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 4, No. 4, 2007, pp. 476 – 480

 en PHYTOCHEMICAL AND IN VITRO ANTIMICROBIAL ASSAY OF THE LEAF EXTRACT OF NEWBOULDIA LAEVIS check for this species in other resources
Usman, H. & Osuji, J. C.

Abstract

The methanolic leaf extract of Newbouldia laevis was subjected to preliminary phytochemical screening and in-vitro antimicrobial tests. The extract revealed the presence of flavonoids, tannins, terpenes, steroidal and cardiac glycosides. The antimicrobial activity of the plant extract was assayed by the agar plate disc diffusion and nutrient broth dilution techniques. Test microorganisms were Pseudomonas aeruginosa check for this species in other resources , Escherichia coli check for this species in other resources , Staphylococcus aureus check for this species in other resources , Salmonella typhi check for this species in other resources , Klebsiella spp. and Candida albicans check for this species in other resources ; all the organisms were laboratory isolates. The extract inhibited the growth of all the test organisms especially against Klebsiella spp. and S. aureus which had mean inhibition zone of 42.3±1.5 and 32.3±1.5 mm respectively. The results showed minimum inhibitory concentration (MIC) of 1.563 mg/ml against Escherichia coli and Klebsiella spp. and 3.125 mg/ml against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. The minimal bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus was 0.39 mg/ml. This study has justified the traditional use of this plant for the treatment of stomach discomfort, diarrhea, dysentery and as a remedy for wound healing whose causative agents are some of the organisms used in this study.

Keywords
Antimicrobial, Leaf Extracts, In vitro, Phytochemical, Newbouldia leavis

 
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