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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 5, No. 2, 2008, pp. 213-222
Bioline Code: tc08030
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 5, No. 2, 2008, pp. 213-222

 en Clerodendron inerme Protects Cellular Integrity During 7,12-dimethylbenz[a]-anthracene induced Hamster Buccal Pouch Carcinogenesis
Shanmugam, Manoharan; Kannan, Kavitha; Subramanian, Balakrishnan & Kasinathan, Rajalingam

Abstract

Aim of the present study was to investigate the protective effect of Clerodendron inerme check for this species in other resources on cellular integrity by measuring the status of glycoconjugates, lipids, osmotic fragility, and membrane bound enzyme activity in 7, 12-dimethylbenz (a) anthracene (DMBA)-induced oral carcinogenesis. Oral squamous cell carcinoma was induced in the buccal pouch of Syrian golden hamsters by painting with 0.5% DMBA in liquid paraffin thrice a week for 14 weeks. The levels of glycoconjugates, lipids, osmotic fragility and membrane bound enzyme activity were analyzed by using specific colorimetric methods. We observed 100% tumor formation in DMBA painted hamsters. Altered glycoconjugates and lipid pattern were observed in DMBA painted hamsters as compared to control hamsters. Erythrocytes from DMBA painted hamsters were more fragile than those from control hamsters. The activity of membrane bound enzyme (Na+ K+ ATPase) decreased in DMBA painted hamsters as compared to control hamsters. Oral administration of aqueous leaf extract of Clerodendron inerme check for this species in other resources (CiALet) at a dose of 500mg/kg body weight significantly prevented the tumor formation and histopathological abnormalities as well as normalized the above said biochemical variables in DMBA painted hamsters. Our results thus demonstrate the protective effect of Clerodendron inerme on cellular integrity during DMBA induced oral carcinogenesis.

Keywords
Oral cancer, DMBA, hamster, Clerodendron inerme, osmotic fragility, lipids, glycoconjugates.

 
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