search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 5, No. 3, 2008, pp. 294-301
Bioline Code: tc08042
Full paper language: English
Document type: Review Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 5, No. 3, 2008, pp. 294-301

 en Influence Of Mitragyna ciliata check for this species in other resources (MYTA) On The Microsomal Activity Of Atpase Na+/K+ Dependent Extract On A Rabbit Heart.
Bidie, Alain dit Philippe; Koffi, Ernest; N’Guessan, Jean-David; Djaman, Allico Joseph & Guede-Guina, Frédérique

Abstract

Mitragyna ciliata check for this species in other resources (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity of the digitalic type on rat’s isolated heart. In this work we studied the effect of MYTA on microsomal Na+/K+ -dependant ATPase (Na+, K+ ATPase) extracted from the heart of a rabbit since digitalics inhibit Na+, K+ ATPase. Our results revealed that the Na+/K+ ATPase has an optimum pH of 7.4 and temperature of 37°C respectively. There is a linear relationship between the organic phosphate formed and the incubation time over 25 mins incubation period. The ATP hydrolysis rate in the presence of MYTA was 0.775 µM/min. LINEWEAVER and BURK plots showed that MYTA did not alter KM (1.31 mM) but decreased VMAX. This study shows that MYTA exerts a non-competitive inhibition on the microsomal Na+/K+ ATPase extracted from rabbit heart with a Ci50 of 48 µg / ml. We conclude that the mechanism of action of MYTA is linked to the inhibition of the Na+/K+ ATPase like cardiotonics of the digitalic type.

Keywords
Mitragyna ciliata; ATPase Na+/K+; inhibitors of ATPase Na+/K+.

 
© Copyright 2008 - African Journal Traditional, Complementary and Alternative Medicines
Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil