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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 5, No. 4, 2008, pp. 363-369
Bioline Code: tc08050
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 5, No. 4, 2008, pp. 363-369

 en Antimicrobial Activity of Various Extracts of Ocimum Basilicum L. and Observation of the Inhibition Effect on Bacterial Cells by Use of Scanning Electron Microscopy
Kaya, Ilhan; Yiğit, Nazife & Benli, Mehlika

Abstract

The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Ocimum basilicum L. were studied. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. The results indicated that the methanol extracts of O. basilucum exhibited the antimicrobial activity against tested microorganisms. While the chloroform and acetone extracts had no effect, the methanol extracts showed inhibition zones against strains of Pseudomonas aeruginosa check for this species in other resources , Shigella check for this species in other resources sp., Listeria monocytogenes check for this species in other resources , Staphylococcus aureus check for this species in other resources and two different strains of Escherichia coli check for this species in other resources . The cells of microorganisms, which were treated and untreated with plant extracts, were observed by using the scanning electron microscope. It was observed that the treated cells were damaged.

Keywords
Antimicrobial activity, Disc diffusion method, Ocimum basilicum, Plant extract, SEM, Spices.

 
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