In this study we designed several pairs of primers to clone and sequence the
ebony gene of a new pigment mutant named black strip (bsr) in
Drosophila melanogaster
(Qian & Zhang, 1994). Compared with
ebony gene of wild type w
91910 and
ebony mutant of e
1, there was no big deletion segment found in coding sequence of
ebony of bsr except for several amino acid mutation sites which did not distribute in the key motif of
ebony protein. However, a big fragment deletion was found at the 5' end of
ebony gene in bsr, which comprised 206 nucleotides of exon 1 and 747 nucleotides of intron 1. Therefore we have been able to accurately localize the mutation locus in bsr.
