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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 27, No. 2, 2006, pp. 181-188
Bioline Code: zr06025
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 27, No. 2, 2006, pp. 181-188

 en Sequence Variability Analysis of MHC Class II DRB Alleles in Three Felines
WANG Qian, WU Xiao-bing, YAN Peng

Abstract

The variation of the exon 2 of the major histocompatibility complex (MHC) class II gene DRB locus in three feline species were examined on clouded leopard ( Neofelis nebulosa check for this species in other resources ), leopard ( Panthera pardus check for this species in other resources ) and tiger ( P. tigris altaica check for this species in other resources ). A pair of degenerated primers was used to amplify DRB locus covering almost the whole exon 2. Exon 2 encodes the β1 domain which is the most variable fragments of the MHC class II molecule. Single-strand conformational polymorphism analysis was applied to detect different MHC class II B haplotypes. Fifteen recombinant plasmids for each individual were screened out, isolated, purified and sequenced. Totally eight sequence haplotypes of exon 2 were obtained in 4 individuals. Within 237 bp nucleotide sequences from 4 samples, 30 variable sites were found, and 21 putative peptide-binding sites were disclosed in 79 amino acid residues. The ratio of nonsynonymous substitutions (dN) was much higher than that of synonymous substitutions (dS), which indicated that balancing selection probably maintain the variation of exon 2. MEGA neighbor joining and PAUP maximum parsimony methods were used to reconstruct phylogenetic trees among species, respectively. The results displayed a more close relationship between leopard and tiger; however, clouded leopard has a comparatively distant relationship from the other two.

Keywords
MHC; DRB locus; Exon 2; Feline; Variability

 
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