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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 29, No. 1, 2008, pp. 17-24
Bioline Code: zr08003
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 29, No. 1, 2008, pp. 17-24

 en Molecular Cloning, Characterization and Expression Analysis of Brain P450arom in Half-smooth Tongue-sole, Cynoglossus semilaevis check for this species in other resources Gunther
DENG, Si-ping; CHEN, Song-lin; LIU, Ben-wei; XU, Jian-yong & TIAN, Yong-sheng

Abstract

The 2184 bp full-length brain P450 aromatase (termed P450aromB) cDNA encoding a protein of 498 amino acid residues was isolated from the brain of the half-smooth tongue-sole ( Cynoglossus semilaevis check for this species in other resources ) by using homologous cloning to clarify the role in sex differentiation. Sequence and phylogenetic analyses showed that the tongue sole P450aromB belonged to brain P450arom subfamilies. The putative P450aromB amino acid residues shared 48.3%-66.1% sequence affinity with other fish brain P450arom, and shared 34.2%-49.9% sequence affinity with ovarian P450arom of other fish. The RT-PCR analysis revealed that P450aromB mRNA was expressed at a high proportion in the brain and gills but less in the gonads and skin, but not in other tissues. However, the P450aromB decreased in the brain of sex-reversed males, treatment by Methyltestosterone or at high temperatures. These results suggested that the P450aromB is involved in the course of gonad development and sex determination in tongue soles.

Keywords
Half-smooth tongue-sole (Cynoglossus semilaevis);P450aromB;cDNA cloning;Expression

 
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