|
Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 30, No. 3, 2009, pp. 341-344
|
Bioline Code: zr09051
Full paper language: Chinese
Document type: Research Article
Document available free of charge
|
|
|
Zoological Research, Vol. 30, No. 3, 2009, pp. 341-344
| en |
Cloning of Cashmere Goat Scp3 Gene and Determining the First Round Meiosis in the Testes
Hou, Yue; Wu, Ying-ji; Luo, Fen-hua; Su, Hui-min; Zhang, Xue-ming; Zhang, Yan; Bai, Yin-ba-tu & Liu, Tao-di
Abstract
The synaptonemal complex protein 3 (Scp3) plays a crucial role in synaptonemal complex formation and male germ cells meiosis. Until now, no studies on goat Scp3 have been reported. In present study, we cloned the Cashmere goat Scp3 cDNA using RT-PCR and molecular cloning techniques. The Scp3 sequence of Cashmere goat showed 98% identity with that of bovine. Primers were designed according to the sequence we got and RT-PCR was performed to analyze the expression of Scp3 in prepubertal Cashmere goat testes. The results showed that the testes development in prepubertal goat varies in different animals, and the first round of meiosis in analyzed Cashmere goat testes occurs mostly after 73 days after birth.
Keywords
Cashmere goat; Synaptonemal complex protein 3; Cloning; Testes development; Meiosis
|
| |
© Copyright 2009 Kunming Institute of Zoology, the Chinese Academy of Sciences
Alternative site location: http://www.zoores.ac.cn/
|
|
