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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 37, No. 4, 2016, pp. 191-204
Bioline Code: zr16030
Full paper language: English
Document type: Review Article
Document available free of charge

Zoological Research, Vol. 37, No. 4, 2016, pp. 191-204

 en The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models
Shao, Ming; Xu, Tian-Rui & Chen, Ce-Shi

Abstract

Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of the targeted gene loci in various cell types and organisms. It can be applied in a number of fields, such as genetic breeding, disease treatment and gene functional investigation. In this review, we introduce the most recent developments and applications, the challenges, and future directions of Cas9 in generating disease animal model. Derived from the CRISPR adaptive immune system of bacteria, the development trend of Cas9 will inevitably fuel the vital applications from basic research to biotechnology and bio-medicine.

Keywords
CRISPR/Cas9; Animal models; Gene therapy

 
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