The coupled model of assimilation and stomatal conductance (
A-gs) was evaluated to estimate leaf stomatal conductance of a drip-irrigated vineyard (
Vitis vinifera
L. cv. Cabernet Sauvignon) located in the Pencahue Valley (35º22’ S, 71°47’ W, 150 m.a.s.l.), Maule Region, Chile, during the 2003-2004 and the 2004-2005 growing seasons. Additionally, a calibration of the three parameters mesophyll conductance (
gm), maximum specific humidity (
Dmax) and coupled factor (
f0) was applied on vines growing in 35 L pots. An infrared gas analyzer was used to calibrate and evaluate the
A-gs which allowed simultaneous measuring of the leaf net CO
2 assimilation (A) and stomatal conductance (
gs) in 2 to 3 h intervals of time. The calibration indicated that the
gm,
Dmax and
f0 values were 1.15 mm s
-1, 52.31 g kg
-1 and 0.90, respectively. The validation in the drip-irrigated vineyard indicated that the
A-gs model was able to estimate the leaf stomatal conductance with a root mean square error (RMSE) of 0.05 mol m
-2s
-1, model efficiency of 61% and agreement index of 90%. The sensitivity analysis indicated that the
A-gs model is affected considerably by the
gm,
Dmax and
f0 parameterization.