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Nucleolar and nucleolus organiser regions activity in tea as visualised by silver staining Wachira, F.N. & Muoki, R.C. Abstract Nucleoli and Nucleolus regions (NORs) in diploid, triploid and tetraploid tea (Camellia sinensis L.) were stained using a simplified technique of silver staining based on the colloidal two-stage method. Nucleoli were visualised in interphase nuclei as black spots against a yellow background. The mode of nucleolar number corresponded to multiples of the somatic cell number and was a good marker for ploidy. Mitotic metaphase chromosomes were stained yellow to golden brown with strongly contrasting NORs appearing as black segments. Keywords Camellia sinensis, silver staining, nucleolus organiser regions

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Wachira, F.N. & Muoki, R.C. Résumé Les zones nucleolees et nucleoles (NORs) dans le the diploide, triploide et tetraploide (Camellia sinensis L.) etaient teintes en se servant d'une technique simplifie d'argentage basee sur la methode collodale a deux stades. Les nucleoles etaient visualisees en interphase de nucleis comme des taches noires sur font jaune. Le mode du nombre nucleolaire correspondait au nombre de cellules somatiques multiples et constituait un bon marqueur de ploidie. Des chromosomes mitotiques en metaphase etaient teintes de couleur jaune or a brune or contrastant avec les NORs qui apparaissent comme de segments noirs. Mots Clés Zones nucleolees actives, the camellia sinensis, argentage

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