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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 17, No. 1, 2014, pp. 6-13
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Bioline Code: ej14002
Full paper language: English
Document type: Research Article
Document available free of charge
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Electronic Journal of Biotechnology, Vol. 17, No. 1, 2014, pp. 6-13
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Segregation distortion in homozygous lines obtained via anther culture and maize doubled haploid methods in comparison to single seed descent in wheat (Triticum aestivum L.)
Adamski, Tadeusz; Krystkowiak, Karolina; Kuczyńska, Anetta; Mikołajczak, Krzysztof; Ogrodowicz, Piotr; Ponitka, Aleksandra; Surma, Maria & Ślusarkiewicz-Jarzina, Aurelia
Abstract
Background: The quality of wheat grain depends on several characteristics, among which the composition of high
molecular weight glutenin subunits, encoded by Glu-1 loci, are the most important. Application of biotechnological
tools to accelerate the attainment of homozygous lines may influence the proportion of segregated genotypes. The
objective was to determine, whether the selection pressure generated by the methods based on in vitro cultures,
may cause a loss of genotypes with desirable Glu-1 alleles.
Results: Homozygous lineswere derived from six winter wheat crosses by pollination with maize (DH-MP), anther
culture (DH-AC) and single seed descent (SSD) technique. Androgenetically-derived plants that originated from
the same callus were examined before chromosome doubling using allele-specific and microsatellite markers. It
was found that segregation distortion in SSD and DH-MP populations occurred only in one case, whereas in
anther-derived lines they were observed in five out of six analyzed combinations.
Conclusions: Segregation distortion in DH-AC populations was caused by the development of more than one
plant of the same genotype from one callus. This distortion was minimized if only one plant per callus
was included in the population. Selection of haploid wheat plants before chromosome doubling based on
allele-specific markers allows us to choose genotypes that possess desirable Glu-1 alleles and to reduce
the number of plants in the next steps of DH production. The SSD technique appeared to be themost advantageous
in terms of Mendelian segregation, thus the occurrence of residual heterozygosity can be minimized by continuous
selfing beyond the F6 generation.
Keywords
Androgenesis; DH lines; HMW glutenin subunits; SSD technique; Winter wheat
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