|
Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 24, No. 1, 2016, pp. 9-11
|
Bioline Code: ej16053
Full paper language: English
Document type: Short Communication
Document available free of charge
|
|
Electronic Journal of Biotechnology, Vol. 24, No. 1, 2016, pp. 9-11
en |
Introgression of LTP2 gene through marker assisted backcross in barley ( Hordeum vulgare )
Mikołajczak, Krzysztof; Ogrodowicz, Piotr; Surma, Maria; Adamski, Tadeusz & Kuczyńska, Anetta
Abstract
Background: Marker-assisted introgression currently represents the most widely spread application of DNA
markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes
that facilitate growth and development under stressful conditions. The homology search against known genes
is a fundamental approach to identify genes among the generated sequences. This procedure can be utilized
for SNP search in genes of predicted function of interest and associated gene ontology (GO).
Results: Backcross breeding enhanced by marker selection may become a powerful method to transfer one or a
few genes controlling a specific trait. In the study, the integrated approach of combining phenotypic selection
with marker assisted backcross breeding for introgression of LTP2 gene, in the background of semi-dwarf
spring barley cultivar, was employed. This study discusses the efficiency of molecular marker application in
backcrossing targeted on the selected gene.
Conclusions: BC6 lines developed in this study can serve as a unique and adequate plant material to dissect the
role of LTP2 gene. Due to its role in lipid transfer, the LTP2 may be crucial in lipidome modification in response
to abiotic stress.
Keywords
BC lines; Gene annotation; Semi-dwarf gene; Single nucleotide polymorphism spring barley; Marker selection; Marker-assisted backcrossing; Marker-assisted introgression; DNA markers; Selection in plant breeding
|
|
© Copyright 2016 - Pontificia Universidad Católica de Valparaíso Alternative site location: http://www.ejbiotechnology.info
|
|