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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 26, No. 1, 2017, pp. 46-51
Bioline Code: ej17021
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 26, No. 1, 2017, pp. 46-51

 en Highly efficient enzymatic preparation of isomalto-oligosaccharides from starch using an enzyme cocktail
Niu, Dandan; Qiao, Jian; Li, Pujun; Tian, Kangming; Liu, Xiaoguang; Singh, Suren & Lu, Fuping

Abstract

Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields.
Results: To improve the process efficiency for production of IMOs,we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis check for this species in other resources pullulanase produced by Bacillus licheniformis check for this species in other resources , α-amylase from Bacillus amyloliquefaciens check for this species in other resources , barley bran β-amylase, and α-transglucosidase from Aspergillus niger check for this species in other resources to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced.
Conclusions: Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use.

Keywords
Aspergillus niger; Bacillus amyloliquefaciens; Bacillus licheniformis; Bacillus naganoensis pullulanase; Barley bran β-amylase; Iisomalto-oligosaccharides production; Saccharification; Ttransglycosylation; α-Amylase; α-Transglucosidase

 
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