Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy
multistage process with low yields.
Results: To improve the process efficiency for production of IMOs,we developed a simple and efficient method by
using enzyme cocktails composed of the recombinant
Bacillus naganoensis
pullulanase produced by
Bacillus licheniformis
, α-amylase from
Bacillus amyloliquefaciens
, barley bran β-amylase, and α-transglucosidase from
Aspergillus niger
to perform simultaneous saccharification and transglycosylation to process the liquefied
starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose,
isomaltotriose, and panose) were produced.
Conclusions: Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive
alternative to the process presently in use.