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Iranian Journal of Pediatrics
Tehran University of Medical Sciences Press
ISSN: 1018-4406
EISSN: 1018-4406
Vol. 17, No. s2, 2007, pp. 277-280
Bioline Code: pe07055
Full paper language: Farsi
Document type: Research Article
Document available free of charge

Iranian Journal of Pediatrics, Vol. 17, No. s2, 2007, pp. 277-280

 en Comparison of Newborn Serum Bilirubin Level with two Methods: Chemical and Direct Spectrophotometric
Joshaghani, Hamid-Reza; Kasalkhe, Nahid & Hajimashadi, Roghieh


Objective: Bilirubin is the endproduct of heme catabolism. Serum bilirubin level can occationally be the only variable that affects the physician's decision. Bilirubin test is one of the most sensitive tests in the clinical laboratory. The aim of this research was to compare results of chemical versus direct spectrophotometric measurement of serum bilirubin.
Material & Methods: This cross-sectional study was carried out in 2006 on 119 specimens of serum. Bilirubin levels were determined by four methods: direct spectrophotometric by DAS and clinic II instruments, chemically with dichloroaniline (DCA), and diazo methods.
Findings: Mean serum bilirubin measured by DAS, clinicII, diazo, and DCA methods was 8.38 (3.59), 7.80 (3.69), 9.70 (4.06), and 8.17 (4.09) mg/dl respectively. Difference of means was significant when examined using ANOVA (P<0.001). The differences between diazo method and the other three methods were significant, while among the latter methods there was no statistically significant difference.
Conclusion: Regarding significant differences between methods, for a better orientation of the physician and more accurate follow up of patients, we recommend to mention the method of measurement in laboratory reports.

Bilirubin , Chemical method , Direct spectrophotometric , Diazo , Newborn

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