Purpose:
To carry out a bioactivity guided fractionation and isolation of the antimicrobial constituent(s) of the roots of
Flemingia strobilifera
against some bacteria and fungi.
Methods:
The root of
F. strobilifera was extracted with methanol, butanol and dichloromethane.
Antimicrobial activity of the extracts was determined against both bacteria and fungi while the
isolation and characterization of compounds of the extracts was done by column
chromatography,2D spectroscopic studies and spectral data (U.V, I.R, NMR and MS).
Results:
Flemingia flavanone
(8, 3'-diprenyl-5, 7, 4'-trihydroxy flavanone), Genistin (5, 4 'dihydroxy isoflavone 7-O-glucoside) and β - sitosterol-D glucoside were isolated from the
extracts.
Flemingia flavanone showed significant antimicrobial activity against Gram-positive (S.
aureus,
S. epidermidis, MRSA), Gram-negative bacteria (
Ps. aeruginosa, E. coli) and fungi (
C.
albicans). Genistin showed moderate activity against Gram-positive, Gram-negative bacteria and
fungi.
Conclusions:
The isolation and antimicrobial activity of presence of
Flemingia flavanone is being reported for the first time.
