Purpose: To isolate high purity pectolinarigenin from
Cirsium Japonicum
and evaluates its antiproliferative
potential and induction of apoptosis activity in MCF-7 breast cancer cell.
Methods: Pectolinarigenin was purified by the combination of silica gel and Sephadex LH-20 column
chromatography. The structure was identified by
1H-nuclear magnetic resonance (NMR),
13C-NMR and
electrospray ionization tandem mass spectrometry (ESI-MS) analyses. The anti-proliferation activity of
pectolinarigenin was also evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and colony
formation assays in MCF-7 breast cancer cell.
Results: Pectolinarigenin showed potent anti-proliferation activity by inducing apoptosis and
downregulation of Bcl2 expression in MCF-7 breast cancer cell. Protein expression of Bcl2 was almost
completely inhibited upon the treatment with 25 µM pectolinarigenin.
Conclusion: A rapid method to isolate high purity pectolinarigenin from
C. japonicum and as well as
evaluate its anti-proliferative potential and induction of apoptosis in MCF-7 breast cancer cells is
presented for the first time. Induction of apoptosis of MCF-7 is via downregulation of Bcl2 expression.