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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 4, 2014, pp. 601-606
Bioline Code: pr14086
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 4, 2014, pp. 601-606

 en Determination of Amino Acids in Medicinal Plants from Southern Sonora, Mexico
Moran-Palacio, Edgar F.; Tortoledo-Ortiz, Orlando; Yañez-Farias, Grelda A.; Zamora-Álvarez, Luis A.; Stephens-Camacho, Norma A.; Soñanez-Organis, José G.; Ochoa-López, Luisa M. & Rosas-Rodríguez, Jesús A.


Purpose: To analyze the amino acid contents of some plants used in traditional medicine in Southern Sonora, Mexico by using high performance liquid chromatography (HPLC).
Methods: The plant samples (Jutuki, Zizyphus obtusifolia check for this species in other resources A.Gray; Jito, Forchammeria watsonii Rose check for this species in other resources ; Barchata, Lycium berlandieri Dunal check for this species in other resources ; Citabaro, Vallesia glabra check for this species in other resources Link; Mangle Rojo, Rhizophora mangle check for this species in other resources L and Tatachinole, Tournefortia hartwegiana check for this species in other resources Steud) were dehydrated at room temperature and ground to a fine powder. Amino acid analysis was performed by reversed-phase HPLC. Elution was carried out with a gradient mobile phase of sodium acetate 0.1 M and methanol (9:1). Samples were derived with ophthalaldehyde (OPA) and detected by fluorescence at 360(Ex)/455(Em) wavelengths.
Results: HPLC analysis resulted in a reliable detection and peak resolution. Tatachinole samples show the greater amino acid concentration (7.83 to 58.17 nM). Fifteen amino acids were detected in plant samples, with aspartic acid (Asp), glutamic acid (Glu), serine (Ser), glycine (Gly), alanine (Ala) and leucine (Leu) (43.55, 44.84, 29.60, 58.17, 43.05 and 38.73 nM, respectively) presenting the highest concentrations.
Conclusion: The amino acids - Asp, Glu, Ser, Gly, Ala and Leu - are naturally involved in osmolyte synthesis, cell metabolism, ammonia detoxification, antioxidant activity and alkaloid synthesis, suggesting that the therapeutic properties of these Southern Sonora plants may have some links to their amino acid composition.

Amino acid profile; Medicinal plants; Osmolytes; Drought/saline stress

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