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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 8, 2014, pp. 1273-1278
Bioline Code: pr14175
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1273-1278

 en Total Glucosides of Paeonia lactiflora check for this species in other resources Pall Suppress Nitric Oxide Production and iNOS Expression in Lipopolysaccharide-Stimulated RAW264.7 Macrophages
Chen, Gang; Tan, Ming-Liang; Gao, Xue & Kong, Shu-Zhen


Purpose: To investigate the effect of total glucosides of Paeonia lactiflora check for this species in other resources (TGPL) on nitric oxide (NO) production and its potential mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells.
Methods: RAW264.7 cells were treated with 10 - 300 μg/ml TGPL and 1 μg/ml LPS. Cell survival was determined by MTT assay. NO level was determined by Griess reaction assay. Inducible NO synthase (iNOS) expression and inhibitor-κBα (IκBα) degradation were determined by Western blot assay. DNA binding activity of NF-κB was determined by ELISA assay using Trans AM™ kit for p65.
Results: The concentrations of TGPL (10 - 300 μg/ml) used in this study did not affect cell survival of RAW264.7 cells, which suggest that 10-300 μg/ml TGPL did not show cytotoxic effect on RAW264.7 cells. NO level and iNOS protein expression significantly increased in LPS-stimulated RAW264.7 cells compared to the unstimulated cells. However, 10 - 300 μg/ml TGPL significantly decreased LPS-induced NO level and iNOS protein expression compared to LPS-stimulated RAW264.7 cells alone. Furthermore, 10 - 300 μg/mL TGPL significantly reduced the content of IκBα protein in LPS-stimulated RAW264.7 cells, which suggests that TGPL inhibited LPS-induced degradation of IκBα protein. TGPL remarkably repressed LPS-induced DNA binding activity of P65 in RAW264.7 cells.
Conclusion: These findings suggest that TGP inhibits NO production and iNOS expression through suppression of NF-κB activation in LPS-stimulated RAW264.7 cells.

Total glucosides; Paeonia lactiflora; Nitric oxide; iNOs; Nuclear factor-κB

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