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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 8, 2014, pp. 1303-1311
Bioline Code: pr14179
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1303-1311

 en Optimized Formation of Benzyl Isothiocyanate by Endogenous Enzyme and its Extraction from Carica Papaya check for this species in other resources Seed
Li, Wen-Zhao; Du, Yi-Deng; Zhang, Yan; Chi, Ying-Zhou; Shi, Zong-Yi; Chen, Wen; Ruan, Mei-Juan & Zhu, Hua-Ping


Purpose: To use endogenous myrosinase in Carica Papaya check for this species in other resources seed to convert benzyl glucosinolate (BG) to benzyl isothiocyanate (BITC) and then extract it for further studies.
Methods: Process variables including seed powder particle size, sample-to-solvent ratio, pH of buffer solution, enzymolysis temperature, enzymolysis time were investigated using single-factor experiments and response surface methodology coupled with Box-Behnken design for further optimization of conversion conditions. The formed benzyl isothiocyanate was extracted by steam distillation method and purified by thin-layer chromatography (TLC). Relevant process variables were also studied. Finally, the purified benzyl isothiocyanate was analyzed by gas chromatography-mass spectrometer (GC-MS) and compared to BITC standard.
Results: Optimum enzymolysis conditions were seed powder particle size, 90 - 120 μm; sample-tosolvent ratio, 1:20; pH of buffer solution, 4.8; enzymolysis temperature, 40°C; and enzymolysis time, 27 min. Enzymolysis liquid was steam-distilled for 2 h, extracted twice using dichloromethane (DCM) (using 3 times the volume of distillate each time) as extractant. Finally, 1:20 was adopted as the optimal dichloromethane-petroleum ether (DCM-PE) elution proportion to wash out the unnecessary compounds.
Conclusion: The result showed that benzyl isothiocyanate was formed under the optimized conditions and was no doubt the basic component of the extraction.

Carica papaya; Benzyl isothiocyanate; Endogenous myrosinase; Enzymolysis; Extraction

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