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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 8, 2014, pp. 1339-1345
Bioline Code: pr14184
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1339-1345

 en Ionic Liquid-Based Ultrasonic/Microwave-Assisted Extraction of Steroidal Saponins from Dioscorea zingiberensis check for this species in other resources C. H. Wright
Wang, Peng; Ma, Chaoyang; Chen, Shangwei; Zhu, Song; Lou, Zaixiang & Wang, Hongxin


Purpose: To develop a more green, efficient and low cost process for the extraction of steroidal saponins from Dioscorea zingiberensis check for this species in other resources C. H. Wright.
Methods: Six kinds of ionic liquids with different cations and anions were evaluated. In addition, the extraction parameters of the ionic liquid based ultrasonic/microwave extraction were studied. In order to quantify the total saponin content accurately, all steroidal saponins were converted into diosgenin and quantified with an appropriate HPLC procedure. IL-UMAE under optimal conditions was compared with heat reflux extraction (HRE) and ultrasonic assisted extraction (UAE).
Results: 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm]BF4) solution at concentration of 0.5 mol L-1 was selected as extraction solvent. The optimum conditions were as follows: solvent to solid ratio 15:1, microwave power 500 W and extraction time 8 min. Under these optimal conditions, the highest extraction yield of diosgenin 10.24 ± 0.31 mg g-1 was obtained. It took only 8 min to obtain the 91.67 % content of diosgenin obtained by traditional HRE. Extracting on time was shortened from 120 min to 8 min.
Conclusion: Compared with traditional methods, IL-UMAE method uses Ionic liquid-solvent which greatly shortens the extraction time. IL-UMAE as a simple, effective and environmentally friendly approach shows a broad prospect for active ingredient extraction.

Dioscorea zingiberensis Steroidal saponins; Ionic liquid; Ultrasonic; Microwave

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