Background: Peltophorum africanum
has been traditionally used to relief stress induced diseases. The study was aimed to evaluate the
antioxidant activities of ethyl acetate extract.
Material and Methods: The
in vitro antioxidant activities of
Peltophorum africanum stem bark extract was examined in this study by means of
+radical scavenging and ferric reducing power analysis using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2`-azino-bis (3-ethylbenzthiazoline-6-
sulfonic acid (ABTS) kit, hydrogen peroxide (H
2O
2), iron (iii) chloride (Fe
3+) and nitric oxide (NO). In assessing the likely effects of secondary
metabolites on the activities observed; total proanthocyanidins, phenolics, flavonols, and flavonoids were determined using standard
phytochemical methods. Data was analyzed by ANOVA test and the
p-value < 0.05 was considered significant.
Results: Extract scavenging activity of 88.73± 6.69 % (25 μg mL
-1), 53.93±1.09 % (25 μg mL
-1), 87.293±6.64 % (25 μg mL
-1), 10.55±2.16 mM
(0.42 mM) and 3.8115±0.06 (25 μg mL
-1) were recorded for H
2O
2, NO, DPPH, ABTS and Fe
3+ reducing power respectively. These values were
comparable to the standard compounds; DBPC*BHT, L (+) - Ascorbic acid and Trolox™ (
p < 0.05). Proanthocyanidins (92.18±4.68 mg/g)
occurred more (
p < 0.05) in the extract when compared to all other compounds tested: phenolics (60.53±1.46 mg/g) > flavonoids (18.37± 2.11
mg/g), > flavonols (11.20±3.90 mg/g). However the difference between flavonols and flavonoids was not significant (
p > 0.05) at 95% confidence
interval.
Conclusion: The results of this study validated the folkloric use of
P. africanum which could be exploited as an easily available and a cheaper
source of natural antioxidants.
