|
African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 14, No. 5, 2017, pp. 181-187
|
Bioline Code: tc17071
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 5, 2017, pp. 181-187
en |
EVALUATION OF THE ANTI-MYCOBACTERIAL, ANTI-OXIDATIVE AND ANTI-PROLIFERATIVE ACTIVITIES OF RUBIA CORDIFOLIA ETHANOLIC LEAF EXTRACT SUB-FRACTIONS IN HUMAN LYMPHOCYTES AND NEUTROPHILS
Makgatho, ME; Nxumalo, W; Ndaba, E; Masilo, C; Tsindane, F & Sedibane, MI
Abstract
Background. Rubia cordifolia has been used to treat various diseases and exhibits antimicrobial, anti-inflammatory,
analgesic and antidiarrheal activities. The plant has not been investigated for its anti-mycobacterial activity against
virulent tuberculosis strains as well as cytotoxic and anti-oxidant activities in mononuclear cells and neutrophils.
Methods. Ethanolic and dichloromethane leaf extract fractions of Rubia cordifolia (0.2- 125μg/ml) were screened for
anti-mycobacterial activity using a fluorescent microplate assay. The anti-oxidant activity of the fractions was tested
using the radical scavenging assay, while their cytotoxicity to lymphocytes was measured using the WST-8 assay.
Reactive oxygen species production by neutrophils exposed to fractions was tested using Cell Meter™ Fluorimetric
ROS Assay kit.
Results. The ethanolic leaf fractions exhibited overall superior anti-mycobacterial activity than the dichloromethane
group and were further screened for anti-oxidant and cytotoxic activities. Ethanolic sub-fractions K2F-3.2, K2F-3.3 and
K2F-3.4 showed significant antioxidant activities at concentration of 50 μg/ml to 200 μg/ml and higher in both the cell
free-based radical scavenging activity and total reactive oxygen species production assays. For lymphocytes, the test
agents showed anti-proliferative activity at 25 μg/ml to 200 μg/ml for sub-fraction K2F-3.3B and 50 μg/ml to 200
μg/ml for agents K2F-3.2 and K2F-3.4.
Conclusion. The current study is the first to record the in vitro anti-mycobacterial, anti-oxidant and cytotoxic activities
of Rubia cordifolia plant leaf extract sub-fractions using the given experimental setups and further research activities to
identify the bioactive components are to be pursued.
Keywords
Rubia cordifolia; DPPH; Mycobacteria; cell proliferation; antioxidant activity; ROS.
|
|
© Copyright 2017 - African Journal of Traditional, Complementary and Alternative Medicines Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam
|
|